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Frequently Asked Questions

Have a question for us? We love hearing from our customers, but if you prefer doing your own research first, here's a list of answers to our customer's most commonly asked questions. If you don't find what you're looking for here, contact our support team using the icon below.

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Biosafety Levels

What is the biosafety level of the microorganism I purchased?

Biosafety levels (BSL) for all our organisms are listed next to the microorganism name in our catalog (both the hard copy and the web page version). All Microbiologics microorganisms are biosafety level (BSL) 1 or 2. The BSL for each strain that Microbiologics provides is listed on the webpage for that strain. BSL 1 organisms have no, or low, risk to individuals or communities. BSL 1 organisms may cause disease in individuals with immune systems that are suppressed or compromised. BSL 2 organisms pose a moderate risk of individual infection, but a low risk of community infection. Guidelines for safety using BSL 1 and 2 microorganisms can be found in this CDC Document.

Certificate of Analysis

How do I get a Certificate of Analysis for the microorganism that I purchased?

A Certificate of Analysis for Microbiologics products can be downloaded on this website. The Certificate of Analysis lists microscopic, macroscopic and phenotypic results for each lot produced at Microbiologics. It also provides proof of traceability to the original Reference Culture. Certificates of Analysis can be downloaded by following the instructions on the Certificate of Analysis page. If you have questions concerning any of the results on the Certificate of Analysis, contact the Technical Support Department.

How do I get a Statistical Analysis Certificate for the microorganism that I purchased?

A Statistical Analysis Certificates can be downloaded by following the instructions on the Certificate of Analysis page. A Statistical Analysis Certificate lists the Mean Assay Value, the Standard Deviation, the Coefficient of Variation, and the 99% and 95% Confidence Intervals obtained by Microbiologics for a quantitative microorganism lot. If you have questions concerning any of the results on a Statistical Analysis Certificate, contact the Technical Support Department.

Certificate of Quality

Where do I locate a copy of the Certificate of Quality?

Certificate of Quality is located on the backside of your shipment's packing slip and also on your account invoice.

Disposal

How do I dispose of the microorganisms that I purchased from Microbiologics?

Check with your local regulatory requirements for proper disposal of biohazard materials. In many cases it is satisfactory to dispose of material in a biohazard container and then use a qualified company to dispose of the biohazardous material.

Expiration Date

What is the expiration date of my product?

The expiration date is on the product’s label.

Growth Promotion Testing

At what temperature should the microorganisms be stored?

The microorganisms should be stored at 2 – 8°C.

How is the Growth Promotion Test performed on new medium?

  • The new medium is inoculated with 100 or fewer CFU of microorganisms.
  • The microorganisms recommended by the pharmacopeia are used in the test. Microorganisms must be no more than 5 passages removed from Reference Culture (the original master seed lot).
  • After incubation, growth on the new batch of medium is compared to growth on the previously approved batch of medium.

  • For further details and illustrated instructions, see the Growth Promotion Test Guides:

    What is the purpose of the Growth Promotion Test?

    The purpose of the Growth Promotion Test is to determine the suitability of culture media to support microbial growth. The medium is challenged with a small number of microorganisms to assure the nutritive properties. The test is described in USP Chapter <61>: Microbiological Examination of Nonsterile Products: Microbial Enumeration Tests, USP Chapter <62>: Microbiological Examination of Nonsterile Products: Tests for Specified Substances and USP Chapter <71>: Sterility Tests.

    What criteria must be met in order for new medium to be accepted for use in tests for specified microorganisms?

    USP Chapter <62>, Microbiological Examination of Nonsterile Products: Tests for Specified Microorganisms, lists the following criteria:

    • The number of colonies on the new agar must be comparable to the number of colonies on the previously approved agar. It is not necessary to be within a factor of two. When testing broth, results should be comparable.
    • Microorganisms which should be inhibited should not grow.
    • Colonies on the new medium should be comparable in appearance and indicative reactions to colonies on previously approved medium.

    How can I ensure good results when I perform a Growth Promotion Test?

    A few of our recommendations for successful Growth Promotion Testing are:

    • Test the new medium and the previously approved medium in parallel.
    • When testing selective medium, test non-selective medium in parallel.
    • Use a spreader to spread the inoculum so that colonies are evenly distributed over the plate.
    • Use a vortex mixer to mix the suspension after delivering each inoculum.

    For more information about using Microbiologics products for Growth Promotion Testing, check out our helpful Growth Promotion Test Guides.

    Where can I find a list of microorganism strains that are available in each product line?

    Visit the Products page or download our current catalog.

    Microbiologics sells four different products for Growth Promotion testing, which one should I choose?

    Use the comparison chart below to determine which product best suits your needs.

    EZ-Accu Shot™ Select

    EZ-Accu Shot™

    EZ-CFU™ One Step

    EZ-CFU ™

    For small volume user For small volume user For medium volume user For large volume user
    Delivers 10-100 CFU/0.1 mlDelivers 10-100 CFU/0.1 mlDelivers 10-100 CFU/0.1 mlDelivers 10-100 CFU/0.1 ml
    No dilution step No dilution step No dilution step One dilution step
    10 tests per vial 10 tests per vial 19 tests per vial >90 tests per vial
    6 vials of hydrating fluid 5 vials of hydrating fluid 10 vials of hydrating fluid 10 vials of hydrating fluid
    Stable for 8 hrs. after hydration, when refrigeratedStable for 8 hrs. after hydration, when refrigerated*Stable for 8 hrs. after hydration, when refrigerated*Must be used within 30 minutes of incubation
    Quick-dissolveQuick-dissolvePre-warm 30 minutes Pre-warm 30 minutes
    Six different strains per kit One strain per kit One strain per kit One strain per kit

    * Exceptions to the 8 hour stability are EZ-Accu Shot, P. aeruginosa, 0484A, and EZ-CFU One Step, C. perfringens, 0318Z and B. fragilis, 0320Z, which must be used within 30 minutes of hydration.

    I need to inoculate my agar with 100 or more CFU. What product should I use?

    Microbiologics has several options available:

    E3 Epower microorganisms contain 1,000 – 9,999 CFU per pellet. In order to deliver 100 CFU or more, hydrate one pellet in 1.0 ml phosphate buffer pH 7.2 and inoculate media with 0.1 ml. 0.1 mL inoculum contains 100-999 CFU. Phosphate buffer pH 7.2 is not provided with this product.

    EZ-CFU microorganisms contain 1,000 – 9,999 CFU per pellet. In order to deliver more than 100 CFU, hydrate two pellets in the 2.0 ml vial of phosphate buffer provided in the canister and inoculate media with 0.1 ml (100-999 CFU).

    Does Microbiologics offer a Verification/Qualification Plan for customers using EZ-Accu Shot™ for the first time?

    Microbiologics offers an EZ-Accu Shot™ Qualification Program for laboratories using EZ-Accu Shot™ products for the first time. The EZ-Accu Shot™ Qualification Program provides EZ-Accu Shot™ pellets from three different lots of each of the five USP compendial strains (15 pellets) as well as a complete guide for qualifying EZ-Accu Shot™ microorganism strains. Contact your Microbiologics sales representative if you would like more information about the program.

    Is the Growth Promotion Test the same in the European Pharmacopoeia and Japanese Pharmacopoeia as in the United States Pharmacopeia?

    The growth promotion test is harmonized in the USP, Ph. Eur., and JP which means the directions are similar for the three pharmacopeias.

    Do I need to test every batch of new media?

    Each new batch of purchased ready-prepared medium, dehydrated medium, and medium prepared from components in the laboratory must be tested.

    What criteria must be met in order for new medium to be accepted for use in microbial enumeration tests?

    USP Chapter <61>, Microbiological Examination of Nonsterile Products: Microbial Enumeration Tests, lists the following acceptance criteria:
    If testing agar, the number of colonies on the new batch of medium must be within a factor of two of the number of colonies on the previously approved medium.  If testing broth, the new and previously approved medium must be comparable in appearance.

    What does it mean to be within a factor of two?

    A factor of two means the number of colonies on the new agar must be no more than twice and no less than half the number of colonies on the previously approved agar. For example, if the number of colonies on the previously approved agar is 40, then the acceptable number of colonies on the new agar is 20-80.

    What if I cannot perform parallel testing because I no longer have the previously approved media?

    If you have performed the growth promotion test on the medium in the past, you can use historical data. Use the same microorganism lot that was used to test the previously approved medium.

    Of course, if you have never performed the growth promotion test before on the type of medium you purchased or made in-house, you have no historical data with which to make a comparison. Microbiologics has two possible solutions to this dilemma:

    • • One, test 3 lots of the medium and compare results. Testing should be done at least in duplicate. All results should meet specifications for recovery and macroscopic characteristics. The results for all 3 lots should be within a factor of two of each other.
    • • Two, test the medium against another brand of ready-made medium from a reputable manufacturer. The media should be tested in parallel and should have the same formulation.

    Please note that the methods listed above are potential solutions. You may want to check with your auditor to see if they have other suggestions.

    Should I test the new batch of media and the previously approved batch of media in parallel in order to compare them?

    Microbiologics strongly recommends using parallel testing. In parallel testing the new medium and the previously approved medium are inoculated with the same microorganism suspension, by the same technician, using the same method and same environmental conditions. The only variable is the medium.

    What should I use to hydrate the lyophilized pellets?

    Use the hydration fluid that came with the kit you received. The amount of hydration fluid in each vial varies between the product lines. The EZ-Accu Shot™ and EZ-Accu Shot™ Select kits include 5 vials with 1.2 ml of hydration fluid in each. EZ-CFU™ and EZ-CFU™ One Step kits include 10 vials of 2 ml of hydration fluid in each. Please note, the hydrating fluid formulation used for Pseudomonas aeruginosa differs from that of other microorganisms.

    Can I use EZ-Accu Shot™, EZ-CFU™ One Step or EZ-CFU™ for Growth Promotion testing of selective media?

    Yes, but also test nonselective media in parallel. Nonselective media is needed as a control because recovery is typically not as high on selective media compared to nonselective media. If 0.1 ml of a microorganism suspension delivers <50 CFU on TSA, the inoculum can be doubled for selective media.

    In the case of cetrimide agar, Microbiologics recommends testing fresh microorganism suspensions prepared from colonies grown from KWIK-STIK Plus, a product that is only two passages from reference culture.

    The medium I am testing is highly selective.I cannot recover microorganisms when I use EZ-Accu Shot™ to inoculate the media with less than 100 cfu. What can I do?

    KWIK-STIK™ Plus two passage microorganisms are ideal for Growth Promotion testing when testing a highly selective medium such as Cetrimide medium. To perform the test, grow the KWIK-STIK™ Plus microorganism on nonselective media. In order to estimate the concentration of an inoculum, use a traditional method such as the turbidimetric method to produce an inoculum of 100 CFU or less.

    Helix Elite Molecular Standards

    Is the Helix Elite™ Synthetic Molecular Standard infectious?

    No. Helix Elite™ Synthetic Molecular Standards are made of synthetic nucleic acid with no human or animal infectious agents.

    Is the sequence of the Helix Elite™ Synthetic Molecular Standard available?

    No. The gene target(s) are listed on the Certificate of Analysis. Please contact Microbiologics Technical Support with questions regarding primer/probe hybridization.

    What volume of Helix Elite™ Synthetic Molecular Standard should I use?

    Typical working volumes should be between 2 µl and 5 µl of diluted Helix Elite™ Synthetic Molecular Standard. Refer to your PCR protocol for exact working volumes.

    Will the Helix Elite™ Synthetic Molecular Standard work with my PCR assay?

    The standard should be compatible with all assays that recognize the target(s). The Molecular Standard is not designed to be exclusive to a specific kit.

    Are Helix Elite™ Synthetic Molecular Standards suitable as spike-in or extraction controls?

    Helix Elite™ Synthetic Molecular Standards are purified nucleic acids NOT intact microorganisms. As such, the stability of nucleic acids in any matrix as well as the sample extraction method should be considered. Organism specific immunological separation steps would not be appropriate since the antigen target is not present.

    Are synthetic Helix Elite Molecular Standards subject to U.S. export controls?

    Yes. Synthetic nucleic acid sequences of specific biological agents listed on the U.S. Commerce Control List are controlled under Export Control Classification Numbers ECCN 1C353. Microbiologics can assist international customers with obtaining the necessary license.

    Are custom-designed Molecular Standards available? Can they be used as internal controls in an in vitro diagnostic assay?

    Yes. Please contact Microbiologics at 320.253-1640 or molecular@microbiologics.com to discuss the synthesis of a new target/sequence for your assay.

    How are Helix Elite™ Synthetic Molecular Standards designed?

    Helix Elite™ Synthetic Molecular Standards contains one or more target sequences that are frequently targeted by molecular assays. The standard is a consensus of available sequences for the target(s).

    What concentration of Helix Elite™ Synthetic Molecular Standard should I use in my assay?

    Typical working concentration should be between 105 CN/reaction and 106 CN/reaction. A higher concentration may be required depending on your instrument and the efficiency of your assay. Refer to the standard curve provided with your Helix Elite™ Synthetic Molecular Standard to determine your recommended working concentration.

    Can the Helix Elite™ Synthetic Molecular Standard be quantified by routine spectrophotometer methods?

    No. The target material is very low mass compared to genomic material (but similar in copy number).
    Copy number/µl is provided with the Certificate of Analysis.

    Inactivated Helix Elite Molecular Standards

    Can I freeze the rehydrated sample?

    Each pellet is intended as a single use test. Dilutions may be performed but should be used immediately. Storage of the rehydrated or diluted material is not recommended.

    How can I get a low titer sample of the same target?

    Each lab should establish their own Ct range using the preferred methods. Based on the determined Ct of each pellet, perform dilutions of the rehydrated pellet to obtain the desired lower concentration.

    Will the Inactivated Helix Elite™ Molecular Standard react with my immunoassay?

    This product has not been tested for use with immunoassays. The product is dispensed at a concentration suitable for the sensitivity of molecular diagnostics, and may not be present in sufficient quantities for detection by other methods. However, the pathogen remains intact following inactivation, and may retain antigenicity.

    How is the product inactivated?

    Organisms are inactivated by heat, chemical or radiation treatment. Each lot of material is tested following treatment to confirm inactivation by looking for infectivity and conducting viability assays (e.g., cytopathic effect or bacterial growth). Universal laboratory precautions are recommended, and material should be handled as though it was a viable specimen.

    What else does the lyophilized pellet contain?

    A proprietary mix of preservatives and excipients (e.g., carbohydrates, gelatin, and buffer) are used to preserve the inactivated material. The pellet dissolves quickly, and is compatible with nucleic acid based testing.

    What is the concentration of the target?

    The concentration of the target is not specified, and should be assessed by each lab according to its protocols. However, each preparation contains "medium-to-high" levels of the target and typically provides a result within a Ct range between 25 and 28 when tested using Microbiologics’ methods.

    What should I use to dissolve the lyophilized pellet?

    The pellet can be dissolved in any relevant biological buffer such as saline, sterile water, or viral transport media.

    How stable is the product?

    Lyophilized pellets are stable when shipped at ambient temperatures, and should be stored at 2°C - 25°C upon receipt. The shelf life is determined based on accelerated and real-time stability studies.

    Instructions For Use

    Where can I find Microbiologics Instructions for Use?

    Instructions for Use and Illustrated Instructions for all Microbiologics products can be found in the Support Hub of this website.

    Lab-Elite

    What strains are available in the Lab-Elite product line?

    Visit the Lab-Elite™ product page of this website for a complete list of strains available as Certified Reference Material. Additional strains will be added as requested.

    What is Certified Reference Material?

    ISO Guide 34:2009 section 3.4 defines reference material as material that is "sufficiently homogenous and stable with respect to one or more specified properties, which has been established to be fit for its intended use in a measurement process.  Properties can be quantitative or qualitative (e.g. identity of substances or species)." Certified Reference Material is accompanied by a Certificate of Analysis.

    How does Microbiologics ensure homogeneity of the Certified Reference Material?

    Microbiologics ensures homogeneity of Lab-Elite™ CRM and Epower™ CRM by analyzing the purity and viability of a representative number of randomly chosen units within each lot produced in accordance with acceptable statistical procedures.

    What are the common uses for Certified Reference Material?

    Method Validation: Use CRMs to validate your test methods, cleaning methods and to validate equipment used for measuring or detecting a substance.

    Method Verification: Verify that your process performs as originally validated.

    Calibration: CRMs that are a pure substance can be used to calibrate equipment intended to detect or measure that substance. CRM can also be used as an independent check of a calibration.

    Method monitoring or process control: Use CRMs to continuously provide evidence that the process is stable. Use CRMs for positive controls. Other reference materials may be adequate for process control.

    Training: Use CRM to train or evaluate the performance of operators. CRM has known identity and property values. Can your operators detect and measure?

    Does Microbiologics sell Certified Reference Material?

    Yes, Microbiologics sells Lab-Elite™ Certified Reference Material (CRM) and Epower CRM. Lab-Elite™ is a pure, homogeneous, stable, lyophilized microorganism preparation with well characterized phenotypic properties. It is accompanied by a certificate which provides values of qualitative properties. Epower™ CRM is a pure, homogeneous, stable, lyophilized microorganism preparation with well characterized phenotypic properties that is available in concentrations ranging from 102 to 108 CFU per pellet.

    Microbiologics became officially recognized as a Reference Material producer in 2009, when the company achieved ISO Guide 34:2000 Accreditation. To achieve Guide 34 accreditation, Microbiologics demonstrated its scientific and technical competence and met the stringent requirements for producing accurate and reliable data.

    What are the ISO 17025 requirements for Certified Reference Material?

    ISO 17025:2005 Section 5.6.3.2 states, "Reference materials shall, where possible, be traceable to SI units of measurement, or to certified reference materials."

    What information does the Lab-Elite Certificate of Analysis provide?

    The Lab-Elite™ Certificate of Analysis provides the microscopic and macroscopic characteristics of the strain as well as the biochemical results obtained in the Microbiologics laboratory.

    How is Lab-Elite packaged?

    Lab-Elite™ packaging is identical to the Microbiologics® KWIK-STIK™. Each Lab-Elite device contains a lyophilized pellet of a single microorganism strain, a reservoir of hydrating fluid and an inoculating swab. The entire Lab-Elite™ kit is packaged inside a canister which includes one Lab-Elite™ CRM device, and a Certificate of Analysis.

    Lyophilization

    What does “lyophilized” mean?

    The term “freeze-dry” is a lay description for the process of lyophilization. It is the conversion of water from a frozen state to a gaseous state without going through a liquid state. The freeze-dry process removes moisture from the cells of specimens while the specimens remain frozen. Microbiologics has uniquely developed the lyophilization process to produce microorganism preparations that are cost-effective and easy-to-use with an excellent shelf-life and dependable quality.

    Mean Assay Value

    Why did I not get the same CFU concentration per pellet as the value printed on the Statistical Analysis Certificate?

    The mean assay value provided on the Statistical Analysis Certificate label is the average assay value obtained by Microbiologics. Specific methods and materials are employed in determining the mean assay value. Your results may differ from the assay value listed on the product’s label due to the use of different materials and methods. However, Microbiologics guarantees that if processed as directed, your results will be within the specific CFU concentration range designated for each individual product line. Please use the chart below for CFU concentration ranges.


    ProductCFU
    EZ-CFU™<100 CFU per 0.1 mL
    EZ-CFU™ One Step<100 CFU per 0.1 mL
    EZ-Accu Shot™<100 CFU per 0.1 mL
    EZ-PEC™ 1.0E+05 to 1.0E+06 CFU per mL of product tested
    EZ-SPORE™ 1.0-9.9E+04 CFU per pellet
    Epower™ and Epower™ CRM

    E2: 1.0-9.9E+02 per pellet
    E3: 1.0-9.9E+03 per pellet
    E4: 1.0-9.9E+04 per pellet
    E6: 1.0-9.9E+06 per pellet
    E7: 1.0-9.9E+07 per pellet
    E8: 1.0-9.9E+08 per pellet

    Microbiologics vs. In-House Cultures

    How is it more efficient to use Microbiologics products over growing and maintaining in-house culture collections?

    There are several advantages to using Microbiologis products rather than growing and maintaining in-house culture collections.

    • • Each Microbiologics product comes with a product warranty stating that each will perform satisfactorily and be free from defect if used and stored as directed.
    • • Each product comes with a built-in safety net. Imagine the time that can be reallocated by moving to a commercial product versus an in-house collection.
    • • Another advantage that makes Microbiologics products efficient is the “hydrate and use” feature. The LYFO DISK® and KWIK-STIK™ products require no pre-incubation or incubation of culture media prior to use.
    • • Using Microbiologics quantitative cultures versus making in-house quantitative suspensions is a time-saver.
    • • Microbiologic products eliminate the need for a -80°C freezer and the high cost associated with that freezer.
    • • A Certificate of Analysis is provided for each product. The certificate provides proof of traceability to the original reference culture.
    • • The risk of contamination is reduced because manipulation of microorganisms is minimized.

    Microorganism Growth

    I am having a problem with my lyophilized microorganism. What should I do?

    First, review the growth requirements for the strain. You can find this information on the strain’s webpage. Under the tab, Culture Method, Microbiologics lists the media, atmospheric conditions, and incubation temperature needed for the strain.

    Second, consult the Product Insert.

    Third, if you need additional assistance, contact the Microbiologics Technical Support Team. Please be prepared to provide the following information:

    • • The nature of the problem
    • • The media on which the organism was grown and the atmosphere and
    • • temperature used
    • • The number of times the organism was sub-cultured, if applicable
    • • The catalog number
    • • The lot number
    • • The number of units involved

    How do I grow my KWIK-STIK™, KWIK-STIK™ Plus, LYFO DISK® and Lab-Elite microorganisms?

    Illustrated Instructions and Product Inserts containing detailed information about growing Microbiologics lyophilized microorganisms are available in the Support Hub of this website.

    If you need to know how to grow a specific strain, you can find the information you need by going to the webpage for that strain. Under the tab, Culture Method, Microbiologics lists the media, atmospheric conditions, and incubation temperature needed for the species.

    The Technical Information Bulletin, Recommended Growth Requirements, also lists the growth requirements for each species that Microbiologics provides. This document can be found in Technical Publications in the Support Hub.

    Broth is not recommended for growing most lyophilized microorganisms because there is a greater risk of contamination.

    For additional assistance, contact the Microbiologics Technical Support Team.

    Microorganism Maintenance

    How do I maintain my microorganism?

    The Microbiologics Technical Information Bulletin titled “Daily QC Microorganism Maintenance” (TIB.246) features detailed instructions for maintaining your microorganisms. This document can be found in the Technical Publications in the Support Hub of this website.

    Passages

    How many passages are my microorganisms from the Reference Culture?

    Lab-Elite™ CRM and Epower™ CRM microorganism preparations are 1 passage from the Reference Culture. A Reference Culture is a microorganism preparation that is acquired from a culture type collection.

    KWIK-STIK™ Plus microorganisms are 2 passages from the Reference Culture.

    KWIK-STIK™ and LYFO DISK® microorganisms are 3 passages or fewer from Reference Culture. The number of passages from Reference Culture is indicated on the Certificate of Analysis.

    All enumerated products are 3 passages or fewer from Reference Culture. The number of passages from Reference Culture is indicated on the Certificate of Analysis.

    Product Inserts

    Where can I find the instructions for use of Microbiologics products?

    Illustrated Instructions and Products Inserts for Microbiologics products can be found in the Document Library on this website.

    Product Label

    How do I find the lot number of my lyophilized microorganism?

    The lot number is on the product label.  It follows the box labeled LOT.

    How do I find the catalog number of my lyophilized microorganism?

    The catalog number is on the product label.  It follows the box labeled REF.

    Purchasing

    Who can buy Microbiologics products?

    Any laboratory that is equipped to handle biohazard material and a legal entity may purchase these products. The lab must be able to agree to the terms in the End User Agreement. These products are not available for purchase by individuals and will not be shipped to a home address. Contact Microbiologics for details.

    QC Slides

    What is on the Blood Parasite Control Slide?

    The Blood Parasite Control Slide, SL90, is an air-dried, methanol-fixed, blood parasite smear containing Plasmodium, Babesia or Trypanosoma.

    A Certificate of Assay is on the inside cover of the box of slides that you purchase.  The certificate identifies the type of parasite on the slides.

    Microbiologics cannot guarantee which parasite you will receive because some blood parasites are not always available. If you need to find out which parasite is on the slide before you make your purchase, contact the Microbiologics Technical Support Team.

    Which parasite is on the Fecal Smear Controls?

    Giardia lamblia cysts are on the fecal smear control slides (catalog numbers SL10-10 and SL15-10).

    How was the Pneumocystis Control Slide made?

    The Pneumocystis control slide (catalog number SL70-10) was made with rat lung tissue and fixed with methanol. We do not guarantee the performance of the slide with fluorescent stains (IDF) but we have found through customer feedback that the Axis-Shield method produces the best results.

    Request Forms

    How can I request a microorganism that is not currently available in your catalog?

    Complete the short, electronic Lyophilized Microorganism Request form to request a microorganism strain or enumerated microorganism preparation that is currently not available from Microbiologics.

    Safety Data Sheets

    Where can I find the SDS for the Microbiologics product I purchased?

    Safety Data Sheets (SDS) for all Microbiologics products can be found in the Support Hub of this website.

    Shipping

    How are the products shipped to my facility?

    Orders are shipped within 48 hours except when a specific shipping date is requested. All shipments are EXW (Ex Works) Saint Cloud, Minnesota, unless prior arrangements are made. Lyophilized microorganisms are identified as Biological Substance Category B or Infectious Substance and are subject to regulated packaging materials, special labeling and special shipping requirements. All shipping and handling charges will be listed as separate line items on the invoice.

     

    Products are shipped at ambient temperature unless otherwise requested. The outside of the shipping container is identified with the notation “refrigerate upon receipt” to assure proper handling and storage upon arrival. Due to the nature of our products, we are unable to accept returns. If any product arrives in a damaged condition, the carrier must note the condition on the delivery receipt. All claims for products damaged during shipment must be made within 30 days of receipt. Microbiologics guarantees the performance of our products at all times. If at any time you are dissatisfied with our products or service, please contact our Customer Service team at 1.320.253.1640 or info@microbiologics.com for resolution.

    Statistical Analysis Certificate

    How do I get a Statistical Analysis Certificate for the microorganism that I purchased?

    A Statistical Analysis Certificates can be downloaded by clicking on the Certificate of Analysis tab at the top of the webpage and then following the instructions. A Statistical Analysis Certificate lists the Mean Assay Value, the Standard Deviation, the Coefficient of Variation, and the 99% and 95% Confidence Intervals obtained by Microbiologics for a quantitative microorganism lot. If you have questions concerning any of the results on a Statistical Analysis Certificate, contact the Technical Support Department.

    Subculturing

    Should I subculture my organism before I test it?

    Yes. The colonies on the pellet plate (the plate on which the rehydrated pellet was grown) may not be fully resuscitated. We do not recommend sub-culturing more than four serial passages from the pellet growth because the organism may mutate. Best practices for phenotypic and susceptibility tests call for testing fresh colonies (24 to 48 hours old). Follow the test manufacturer’s instructions.

    UV-BioTAG

    What equipment do I need to see a UV-BioTAG culture fluoresce?

    You need a UV lamp and a dark room. UV-BioTAG strains are best visualized using the following wavelengths:

    Microorganism Strain

    UV Light Wavelength

    Escherichia coli (O157:H7) EC43 (STEC)315 – 400 nm
    Listeria innocua (6a)*475 – 495 nm
    Listeria monocytogenes (1/2a)*475 – 495 nm
    Listeria monocytogenes (1/2b)*475 – 495 nm
    Listeria monocytogenes (4b)*475 – 495 nm
    Salmonella enterica subsp. enterica serovar Typhimurium Sal54315 – 400 nm
    Salmonella enterica subsp. enterica serovar Senftenberg Sal59315 – 400 nm
    Shigella flexneri SF84315 – 400 nm

    *Listeria strains are best visualized using blue light barrier lenses.

    Which Microbiologics strains are available in UV-BioTAG?

    The following UV-BioTAG strains are available:

    Microorganism Strain

    Source

    Catalog # 6-Swab Kit

    Catalog # 6-Vial Kit

    Escherichia coli (O157:H7) EC43 (STEC)Derived from FDA ESC117701227UV-S01227UV-V
    Listeria innocua (6a)Modified from NCTC 1128801271UV-S01271UV-V
    Listeria monocytogenes (1/2a)Derived from FDA LS80801249UV-S01249UV-V
    Listeria monocytogenes (1/2b)Derived from FDA LS81001248UV-S01248UV-V
    Listeria monocytogenes (4b)Derived from FDA LS80601250UV-S01250UV-V
    Salmonella enterica subsp. enterica serovar Typhimurium Sal54Derived from FDA SAL569401223UV-S01223UV-V
    Salmonella enterica subsp. enterica serovar Senftenberg Sal59Derived from FDA SAL569701226UV-S01226UV-V
    Shigella flexneri SF84Derived from FDA SHI055201224UV-S01224UV-V

    On which agars will UV-BioTAG microorganisms fluoresce?

    UV Bio-TAG strains fluoresce on the following agars:

    Microorganism Strain

    Catalog # 6-Swab Kit

    Growth

    UV Wavelength

    Escherichia coli (O157:H7) EC43 (STEC)01227- Tryptic Soy Agar
    - Tryptic Soy Agar with 5% Sheep Blood Agar
    - LB Agar with Chloramphenicol
    - MacConkey Sorbitol
    - Hektoen Enteric (HE) Agar
    - Xylose Lysine Deoxycholate (XLD) Agar
    315 - 400 nm
    Listeria innocua (6a)01271- Tryptic Soy Agar with 5% Sheep Blood Agar

    475-495 nm (use simultaneously with blue light barrier glasses)
    Listeria monocytogenes (1/2a)
    Listeria monocytogenes (1/2b)
    Listeria monocytogenes (4b)
    01249
    01248
    01250
    - Tryptic Soy Agar
    - Tryptic Soy Agar with 5% Sheep Blood Agar
    - Modified Oxford (MOX) Agar
    - ALOA Agar (Selective chromogenic medium for the detection of Listeria)
    475-495 nm (use simultaneously with blue light barrier glasses)
    Salmonella enterica subsp. enterica serovar Typhimurium Sal5401223- Tryptic Soy Agar
    - Tryptic Soy Agar with 5% Sheep Blood Agar
    - LB agar with Chloramphenicol
    - Hektoen Enteric (HE) Agar
    - Xylose Lysine Deoxycholate (XLD) Agar
    315 - 400 nm
    Salmonella enterica subsp. enterica serovar Senftenberg Sal5901226- Tryptic Soy Agar
    - Tryptic Soy Agar with 5% Sheep Blood Agar
    - LB Agar with Chloramphenicol
    - Hektoen Enteric (HE) Agar
    - Xylose Lysine Deoxycholate (XLD) Agar
    315 - 400 nm
    Shigella flexneri SF8401224- Tryptic Soy Agar
    - Tryptic Soy Agar with 5% Sheep Blood Agar
    - LB Agar with Chloramphenicol
    - Hektoen Enteric (HE) Agar
    - Xylose Lysine Deoxycholate (XLD) Agar
    315 - 400 nm

    How is UV-BioTAG packaged?

    UV-BioTAG is available in two formats:

    • UV-BioTAG Swab kits include six swabs, each containing one lyophilized pellet of an individual microorganism strain

    • UV-BioTAG Vial kits include six vials, each containing one lyophilized pellet of an individual microorganism strain.

    Where can I find the Instructions for Use (Product Insert), Safety Data Sheets (SDS) and Certificates of Analysis?

    Instructions for Use, The Safety Data Sheets, and Certificates of Analysis can be found in the Support Hub.

    Can I freeze UV-BioTAG?

    Microbiologics does not guarantee a UV-BioTAG strain will retain its unique characteristics if it is frozen.

    What are the components of UV-BioTAG?

    A UV-BioTAG lyophilized preparation consists of:

    • • The microorganism population
    • • Skim milk (Bovine – USA origin)
    • • Carbohydrate
    • • Gelatin (Porcine – USA or Canada origin)
    • • Ascorbic acid

    How can I use UV-BioTAG for quality control of tests for pathogen detection?

    • • Grow the microorganism on nonselective agar
    • • Inoculate enrichment broth with the UV-BioTAG strain
    • • Subculture the broth to Tryptic Soy Agar or another agar which it is known to fluoresce on
    • • Check for fluorescence

    For more details see the Instructions for Use.

    Will UV-BioTAG microorganisms retain their fluorescence when they are subcultured?

    UV-BioTAG microorganisms will retain their fluorescence for one month if the Microbiologics Microorganism Maintenance Plan is followed. For best results start a new culture with each test.

    Are all media and test processes suitable for UV-BioTAG?

    Some processes or matrices may produce biochemical by-products that mask fluorescence. Also some broths and agars emit fluorescence that may mask UV-BioTAG fluorescence. Check your media for fluorescence before inoculating with UV-BioTAG.

    Warranty

    Does Microbiologics warrant its products?

    Yes, if the product is used as intended, stored as directed, processed in accord with instructions and used before the expiration date. For more information see Microbiologics "Product Warranty and Product Replacement".