LEADING THE WAY IN ANTIVIRAL TESTING SERVICES
With the persistence of infectious disease cases globally, research into antiviral prevention and treatment remains one of the most critical public health concerns today. At Microbiologics, we are committed to sharing our resources and expertise to support researchers and developers in their quest to discover novel, life-changing antiviral agents. As a trusted partner to bio/pharmaceutical companies worldwide, we offer a comprehensive range of in vitro antiviral testing services designed to meet your research needs.
From standardized, off-the-shelf assays to custom designed assays, we're committed to getting your antiviral studies off the ground and running with unmatched precision and efficiency.
EXPLORE OUR OFF-THE-SHELF ANTIVIRAL ASSAYS
We understand the importance of speed and reliability in antiviral research. That's why we’ve streamlined our assay offerings, making it easier and quicker for you to access the tests you need. With our newly standardized assays, you can expect the same high-quality data with reduced turnaround times, allowing you to make informed decisions and move projects forward faster.
Hemagglutination Inhibition (HAI) Assay
Our HAI assay is a go-to standard for evaluating the efficacy of influenza vaccines and the concentration of antibodies able to inhibit the influenza HA protein. This assay utilizes viral agglutination of RBCs by a specific antibody, providing critical data for virus antibody response and quantitative antibody titers.
HAI Reference Strains
| Virus | Isolate |
|---|---|
| H1N1 | A/PuertoRico/08/1934 |
| A/California/7/2009 | |
| A/Michigan/45/2015 | |
| A/Wisconsin/588/2019 | |
| A/Victoria/4897/2022 | |
| H3N2 | A/Perth/16/2009 |
| A/Texas/50/2012 | |
| A/Switzerland/9715293/2013 | |
| A/Hong Kong/4801/2014 | |
| A/Darwin/6/2021 | |
| A/Darwin/9/2021 | |
| B Yamagata | B/Massachusetts/2/2012 |
| B/Phuket/3073/2013 | |
| B Victoria | B/Malaysia/2506/2004 |
| B/Brisbane/60/2008 | |
| B/Austria/1359417/2021 | |
| B/Wisconsin/1/2010 |
Our Off-the-Shelf HAI Assay Includes
- Options for samples to be tested in either single, duplicate, or triplicate against a panel of 4 influenza viruses
- Choice of monoclonal antibody or serum sample types
- Serum is RDE treated, and tested from 1:10 to 1:1280 dilutions
- Antibody samples will be tested at a defined starting concentration of 8 two-fold dilutions
- Positive serum controls for all reference test strains
- A comprehensive report with methodology and test results for each sample
NEURAMINIDASE (NAI) ASSAY (ELLA)
The NAI ELLA assay is essential for measuring antibody titers against influenza neuraminidase (NA) protein. It measures the optical density from the enzymatic cleavage of sialic acids from fetuin by NA. The reciprocal of the highest dilution that results in ≥50% inhibition of NA activity is designated as the NI antibody titer.
NAI ELLA Reference Strains
| Virus | Isolate |
|---|---|
| H1N1 | A/California/7/2009 |
| A/Wisconsin/588/2019 | |
| A/Michigan/45/2015 | |
| H3N2 | A/Perth/16/2009 |
| A/Texas/50/2012 | |
| A/Hong Kong/4801/2014 | |
| A/Darwin/6/2021 | |
| A/Darwin/9/2021 | |
| B Yamagata | B/Massachusetts/2/2012 |
| B/Phuket/3073/2013 | |
| B Victoria | B/Malaysia/2506/2004 |
| B/Austria/1359417/2021 | |
| B/Wisconsin/1/2010 | |
| B/Brisbane/60/2008 |
Our Off-the-Shelf NAI ELLA Assay Includes
- Serum will be heat inactivated, sub aliquoted and frozen into single use testing vials
- Antibody samples will be thawed, sub aliquoted and frozen into single use testing vials
- Either the Serum or Antibody samples will be diluted to appropriate starting dilution or concentration (i.e. ug/mL) and serially diluted three-fold out to 8 dilutions
- The default starting dilution for serum is 1:40 dilution and for Antibody is 100ug/mL
- An amount of influenza virus will be mixed with the test sample that corresponds to 90% NA Activity which been pre-determined prior to testing
- Samples will be tested in triplicate
- OD values will be normalized using nonlinear regression inhibition vs. response variable slope (four parameters) analysis to calculate the either the IC50 value or inhibition results for each test sample
- A comprehensive report with methodology and test results for each sample
INFLUENZA MICRONEUTRALIZATION (MN) ASSAY
Our Influenza MN assay is designed to evaluate the neutralization of antibodies by measuring their ability to inhibit virus infection. This cell-based, standardized assay provides reliable, reproducible data crucial for your research.
Influenza MN Reference Strains
| Virus | Isolate |
|---|---|
| H1N1 | A/Wisconsin/588/2019 |
| H3N2 | A/Darwin/9/2021 |
| B Yamagata | B/Phuket/3073/2013 |
| B Victoria | B/Austria/1359417/2021 |
| B/Malaysia/2506/2004 |
Our Off-the-Shelf MN Assay Includes
- Serum will be heat inactivated, sub aliquoted and frozen into single use testing vials
- Antibody samples will be thawed, sub aliquoted and frozen into single use testing vials
- Serum samples will be diluted to 1:40 dilution and serially diluted two-fold out to 8 dilutions
- Antibody samples will be diluted to appropriate starting dilution or concentration (i.e. ug/mL) and serially diluted two-fold out to 8 dilutions
- The default starting dilution for serum is 1:40 dilution and for Antibody is 100ug/mL
- All samples will be tested in duplicate
- A constant amount of influenza virus will be mixed with each test sample
- Antibody or serum sample and virus will be pre-incubated for 1 hour then added to cells
- The resulting antibody titer will be provided based on the lowest concentration (most dilute) antibody concentration or serum dilution that inhibited virus replication based on ELISA readout in order to determine the end point neutralization titer
- A comprehensive report with methodology and test results for each sample
SARS-CoV-2 MICRONEUTRALIZATION ASSAY
We offer a high-throughput, validated, ELISA-based Microneutralization Assay for SARS-CoV-2* virus for late-stage clinical development. We can also develop custom viral assays for SARS-CoV-2, influenza, and many other viruses.
*Microbiologics is a licensed service provider of this assay through the Battelle Memorial Institute.
EXPLORE OUR CUSTOM ANTIVIRAL ASSAYS
We understand that antiviral research often requires unique solutions. Our scientists possess deep virological expertise and are adept at designing and optimizing custom assay programs for a wide range of viruses. We stay at the forefront of assay technology, incorporating the latest advancements to deliver cutting-edge solutions. We are skilled in technology transfers to facilitate the passing of knowledge, documentation, and protocols between our laboratories and yours.
Custom Antiviral Assays Offered:
- Inhibition of infection
- Titer reduction assay
- Plaque assay
- Plaque inhibition assay
- Neutralization of infectivity by antibody or antibody like molecules
- TCID50 assay
- IC50 Assay
- Hemagglutination Assay
- Textiles and Plastics Assay (AATP)
Ready to Accelerate Your Antiviral Research?
We're here to support your antiviral studies every step of the way. Whether you're ready to start with our standardized assays or looking to customize an assay program to suit your project, our Business Development team is ready to help you find the perfect fit for your research needs.
Business Development Team
Phone: +1.800.599.2847
Email: businessdevelopment@microbiologics.com