LYFO DISK™
Catalog No. 01026L
Desulfotomaculum nigrificans derived from ATCC® 7946™*
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1 Vial
General Information
Culture Method
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General Information
Biosafety Level1
Product FormatLYFO DISK™
Test MethodMicrobial Identification
Catalog number01026
TaxonomyBacteria
Price CodeL-E
Culture Method
MediaPhase 1: ISF (modified Infant Soy Formula) Broth, Phase 2: Tranfer to Sulfite Agar
TemperaturePhase 1: 55°C, Phase 2: 55°C
AtmospherePhase 1: Anaerobic, Phase 2: Anaerobic
Growth TimePhase 1: 48 hours, Phase 2: 48 hours to 7 days
Additional TipsPrepare and Use ISF (modified Infant Soy Formula) Broth using the following steps:
1. Fill tubes with 10 ml Infant Soy Formula. Infant Soy Formula may be purchased at a grocery store.
2. Place a four-penny nail in each tube. A four-penny nail is approximately 1.5 inches, or 38 mm, in length. It should contain steel or iron.
3. Sterilize the broth.
4. Inoculate ISF Broth with one LYFO DISK® or KWIK-STIK™.
5. Grow at 55°C in anaerobic conditions for 48 hours. The broth will turn grey, indicating growth.
6. Make two dilutions, 1:10 and 1:100.
7. Sub with a swab to Sulfite Agar. Plate the undiluted sample and the 1:10 and 1:100 dilutions. It is necessary to plate the diluted samples because at higher concentrations the colonies are pin-point which makes colony characteristics difficult to see. Sulfite Agar is used for detecting thermophilic anaerobes which produce sulfite.
8. Incubate the agar in anaerobic environment at 55°C for 48 hours to 7 days.
1. Fill tubes with 10 ml Infant Soy Formula. Infant Soy Formula may be purchased at a grocery store.
2. Place a four-penny nail in each tube. A four-penny nail is approximately 1.5 inches, or 38 mm, in length. It should contain steel or iron.
3. Sterilize the broth.
4. Inoculate ISF Broth with one LYFO DISK® or KWIK-STIK™.
5. Grow at 55°C in anaerobic conditions for 48 hours. The broth will turn grey, indicating growth.
6. Make two dilutions, 1:10 and 1:100.
7. Sub with a swab to Sulfite Agar. Plate the undiluted sample and the 1:10 and 1:100 dilutions. It is necessary to plate the diluted samples because at higher concentrations the colonies are pin-point which makes colony characteristics difficult to see. Sulfite Agar is used for detecting thermophilic anaerobes which produce sulfite.
8. Incubate the agar in anaerobic environment at 55°C for 48 hours to 7 days.
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Documentation
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*The ATCC Licensed Derivative Emblem, the ATCC Licensed Derivative word mark and the ATCC catalog marks are trademarks of ATCC. Microbiologics, Inc. is licensed to use these trademarks and to sell products derived from ATCC® cultures. Look for the ATCC Licensed Derivative © Emblem for products derived from ATCC© cultures.