LYFO DISK™
Catalog No. 01026L
Desulfotomaculum nigrificans derived from ATCC® 7946™*
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1 Vial
General Information
Culture Method
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General Information
Biosafety Level1
Product FormatLYFO DISK™
Test MethodMicrobial Identification
Catalog number01026
TaxonomyBacteria
Price CodeL-E
Culture Method
MediaPhase 1: ISF (modified Infant Soy Formula) Broth, Phase 2: Tranfer to Sulfite Agar
TemperaturePhase 1: 55°C, Phase 2: 55°C
AtmospherePhase 1: Anaerobic, Phase 2: Anaerobic
Growth TimePhase 1: 48 hours, Phase 2: 48 hours to 7 days
Additional TipsPrepare and Use ISF (modified Infant Soy Formula) Broth using the following steps:
1. Fill tubes with 10 ml Infant Soy Formula. Infant Soy Formula may be purchased at a grocery store.
2. Place a four-penny nail in each tube. A four-penny nail is approximately 1.5 inches, or 38 mm, in length. It should contain steel or iron.
3. Sterilize the broth.
4. Inoculate ISF Broth with one LYFO DISK® or KWIK-STIK™.
5. Grow at 55°C in anaerobic conditions for 48 hours. The broth will turn grey, indicating growth.
6. Make two dilutions, 1:10 and 1:100.
7. Sub with a swab to Sulfite Agar. Plate the undiluted sample and the 1:10 and 1:100 dilutions. It is necessary to plate the diluted samples because at higher concentrations the colonies are pin-point which makes colony characteristics difficult to see. Sulfite Agar is used for detecting thermophilic anaerobes which produce sulfite.
8. Incubate the agar in anaerobic environment at 55°C for 48 hours to 7 days.
1. Fill tubes with 10 ml Infant Soy Formula. Infant Soy Formula may be purchased at a grocery store.
2. Place a four-penny nail in each tube. A four-penny nail is approximately 1.5 inches, or 38 mm, in length. It should contain steel or iron.
3. Sterilize the broth.
4. Inoculate ISF Broth with one LYFO DISK® or KWIK-STIK™.
5. Grow at 55°C in anaerobic conditions for 48 hours. The broth will turn grey, indicating growth.
6. Make two dilutions, 1:10 and 1:100.
7. Sub with a swab to Sulfite Agar. Plate the undiluted sample and the 1:10 and 1:100 dilutions. It is necessary to plate the diluted samples because at higher concentrations the colonies are pin-point which makes colony characteristics difficult to see. Sulfite Agar is used for detecting thermophilic anaerobes which produce sulfite.
8. Incubate the agar in anaerobic environment at 55°C for 48 hours to 7 days.
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